Ultralong Tracking of Fast‐Diffusing Nano‐Objects inside Nanofluidic Channel−Enhanced Microstructured Optical Fiber

Nanoparticle tracking analysis (NTA) represents one essential technology to characterize diffusing nanoscale objects. Herein, uncovering dynamic processes and high-precision measurements requires tracks with thousands of frames reach high statistical significance, ideally at frame rates. Optical fibers nanochannels are used for NTA, successfully demonstrating acquisition trajectories fast diffusion nano-objects 100 000 frames. Due the spatial limitation central nanofluidic channel, objects illuminated by core mode is confined, enabling recording Brownian motion over extraordinarily long time scales The resulting benefits discussed on a representative track gold nanosphere in water nearly 2 kHz rate. In addition verification fiber-based NTA using two data processing methods, segmented reveals correlation between precision determined diameter continuous interval (i.e., number per subtrajectory). presented results demonstrate capabilities terms 1) determining diameters extraordinary single species 2) monitoring object or fluidic environment, both which relevant within biology, microrheology, nano-object characterization.